Undergraduate Education of Food Science and Technology,TUST
Course Description
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Food Microbial Detection Technology

Course Code: PRC3104
Credits: 2
Teaching Hour: 40 (16 hours for lecture, 24 hours for laboratory)
Prerequirement: Microbiology, Biochemistry
Instructor: Prof Chunling Wang

Course Objective: To extend the basic concepts and techniques in Microbiology to food safety in processing and distribution, esp., to detection of pathogenic and spoilage microbial.

Main contents:
Chapter 1 Introduction
1.1The concepts and characteristics of food microbial detection
1.2 The development history and progress of microbial detection
Chapter 2 Microbial detection technology
2.1 Basic knowledge of microbiological detection
2.2 Conventional techniques for food microbial detection
2.3 Food microbiology detection technology
2.4 Food microbial molecular biology
2.5 Analytical microbial detection technology
Chapter 3 Collection and pretreatment of food detection sample
3.1 Principles of food detection sample collection
3.2 Food detection sample collection method
3.3 Food detection and handling of samples
Chapter 4 Index of food microbial detection
4.1 Total colonies
4.2 MPN of Escherichia coli
4.3 Pathogenic microorganisms
Chapter 5 Detection of poison microorganisms in foods
5.1 Salmonella test
5.2 Staphylococcus aureus test
5.3 Clostridium botulinum test
5.4 Mould and yeast count
Lecture Hour Allocate
Chapter
Title
Hour
1
Introduction
2
2
Microbial detection technology
2
3
Collection and pretreatment of food detection sample
4
4
Index of food microbial detection
4
5
Detection of poison microorganisms in foods
4
Total
 
16

Laboratory Hour Allocate
Laboratory
Title
Hour
1
Sample collection, preparation, pretreatment and preservation
2
2
Determination of total count and Escherichia coli in water
4
2
Detection of yeast and mold in cake
4
3
Detection of Salmonella in eggs
4
4
Detection of Clostridium botulinum in canned foods
4
5
PCR Detection of Staphylococcus aureus in milk
4
6
ELISA detection of alfatoxin in corn
2
Total
 
24


Assessment：2 hour written examination at end of the course (40%)；Laboratory report (60%).
Qualifying standard: 60%
Penalties: Absence of examination and laboratory and any academic misconduct shall be assigned a mark of zero.
Text book: Du Lianxiang, Lu Fuping. Microbiology experimental technology, China Light Industry Press, 2006
References
1.Shen Ping, Fan Xiurong, et al. Microbiology experiment, 3rd ed, Higher Education Press, 2000
2.Zhao Bin, He Shaojiang. Microbiology experiment, 1st ed. Science Press, 2002